Composite

Part:BBa_K1615061:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


CBDcex fused to Heroin Esterase driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1998
    Illegal BamHI site found at 2754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

Heroin Esterase from Rhodococcus erythropolis. CBDcex from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington.

References